The main aim of our project is to identify the role of ASPH in tumors of head and neck, which serves as a model for comparison of the tumors of the same anatomical location with different etiology, in normoxic and hypoxic conditions. One of the partial aims is the identification of the genes influenced by ASPH expression. It allows to identify unknown targets and mechanisms through which ASPH contributes to the malignant phenotype of the cells. Human ASPH catalyzes post-translational hydroxylation of aspartyl and asparaginyl residues in domain resembling epidermal growth factor (EGF). ASPH is important for migration and invasiveness of cells in the natural conditions (trophoblast of placenta) but also in tumors. It has been discovered that one of the mechanisms by which ASPH influences the motility of cells is activation of the Notch signaling pathway. The increase of migration and invasiveness of cells in response to the increased production of ASPH leads to the degradation of extracellular matrix. However, the principal mechanisms of these processes were not described yet. Furthermore, the EGF-like domain is also contained in a number of other proteins besides Notch and nothing is known about the influence of ASPH on their activity. Therefore, we will focus on identification of genes influenced by ASPH expression in normal and immortalized keratinocytes. Additionally, also genes whose expression is influenced by ASPH will be identified in cell lines derived from head and neck tumors of different etiology.
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Koslabova E, Hamsikova E, Salakova M, Klozar J, Foltynova E, Salkova E, Rotnaglova E, Ludvikova V, Tachezy R. Markers of HPV infection and survival in patients with head and neck tumors. Int J Cancer, 133, 8, 1832-9, 2013. IF 6,513
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Phenotype and function of head and neck squamous carcinoma immune cells as predictive markers of clinical response, 2017-2022, AZV 17-28255A
The role of aspartyl beta-hydroxylase in tumors associated and non-associated with human papillomaviruses, 2019-2022, Inter-Excellence, sub-program Inter-Action LTAUSA18003
Center for Tumor Ecology – Research of the Cancer Microenvironment Supporting Cancer Growth and Spread, 2018-2023, Excellent Research, MŠMT CZ.02.1.01/0.0/0.0/16_019/0000785
Metagenomic analysis of the virome of the honeybee (Apis mellifera) in the Czech Republic, 2017-2019, CELSA (Central Europe Leuven Strategic Alliance)
The development of MULTIOMICS pesticide risk assessment for bees with regard to real contamination and other stressors, 2019-2023, QK – Program of applied research Ministry of agriculture, Earth, QK1910018
The role of aspartyl beta-hydroxylase in tumors associated and non-associated with human papillomaviruses, 2019-2022, Inter-Excellence, sub-program Inter-Action LTAUSA18003
Deadline is closed